Strain screening is one of the most common categories of bioprocess work that is carried out at Culture. Fundamentally, the types of screening processes that can be run in Culture’s cloud bioreactors are no different than those that could be performed at other facilities. However, there are a few advantages to screening strains at Culture.
Variety of adaptive control schemes
Culture has a variety of fermentation process control methods that can be incorporated into screening processes. These include automated feed triggers and dynamic control during the fed-batch based on parameters such as pH, OUR or RQ. Customers can specify an adaptive control scheme, and Culture will build it into the bioprocess recipe.
Culture’s high-throughput bioreactor capacity means that there does not need to be a tradeoff between structuring replicates based on statistics and the throughput of strains. Culture’s capacity enables screening larger numbers of strains in a shorter period of time.
Precision fermentation control
Each of Culture’s reactors is equipped with several features to ensure precision fermentation control, reducing biological variability as a consequence of imprecise process control. Lower biological variability between replicates can also increase the statistical confidence in results. These features include gases delivered via mass flow controllers and up to 5 feeds delivered via peristaltic pump with scale feedback control. Published mass balance data on every run
also ensures quality data.
Accessible data and time to analyze it
Culture’s Cloud Console allows you to design, monitor and analyze experiments from your laptop. Console enables access to data from single or multiple runs and allows for grouping and graphing of data for easy analysis. Graphs can be saved and shared in workspaces to allow rapid analysis of strain performance and collaboration across groups. In addition, as Culture manages the execution of experiments, time can be spent analyzing data rather than troubleshooting reactors.